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Methods. 2007 Aug;42(4):321-4.

Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals.

Author information

1
School of Biomedical Sciences, University of Ulster at Coleraine, Cromore Road, Coleraine BT52 1SA, Co. Londonderry, Northern Ireland, UK. s.sarker@ulster.ac.uk

Abstract

The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds against various bacterial strains. This modified resazurin method is simple, sensitive, rapid, robust and reliable, and could be used successfully to assess antibacterial properties of natural products.

PMID:
17560319
PMCID:
PMC1895922
DOI:
10.1016/j.ymeth.2007.01.006
[Indexed for MEDLINE]
Free PMC Article

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