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Arch Oral Biol. 2007 Nov;52(11):1109-11. Epub 2007 Jun 7.

Effect of iron on acid demineralisation of bovine enamel blocks by a soft drink.

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Social Dentistry Department, Bauru School of Dentistry, University of São Paulo, Bauru, Al. Octávio Pinheiro Brisolla, 9-75 Bauru, SP 17012-901, Brazil.



The aim of this study was to evaluate, in vitro, the effect of iron on the acid demineralisation of bovine enamel blocks by a soft drink.


Twenty-four blocks of bovine enamel were randomly assigned to two groups (experimental and control), which were exposed to 4 cycles of demineralisation in Coke containing or not 10 mmol/L of iron, respectively, and another 4 cycles of remineralisation in artificial saliva. The softening of enamel was evaluated by % superficial microhardness change (SMHC) and wear analysis. Data were analysed using t-test (p<0.05).


With regard to %SMHC, the experimental group presented average values (+/-S.E.) of -85.3+/-0.9, that were significantly higher (t=4.7, p<0.01) than the ones observed for control group (-76.7+/-1.6). The experimental group presented a mean wear (+/-S.E.) of 2.3+/-0.2 microm, that was significantly lesser (t=3.7, p<0.01) than the one observed for the control group (3.5+/-0.2 microm).


According to this protocol, iron at 10 mmol/L significantly reduced the wear, but significantly enhanced the %SMHC of enamel blocks submitted to erosion by Coke.

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