Mutations of the RAG3 gene encoding a regulator of fermentation in Kluyveromyces lactis are suppressed by a mutation of the transcription factor gene KlGCR1

FEMS Yeast Res. 2007 Aug;7(5):675-82. doi: 10.1111/j.1567-1364.2007.00219.x. Epub 2007 Jun 9.

Abstract

In Kluyveromyces lactis, Rag3 regulates both fermentative metabolism and thiamine biosynthesis. Regulation of fermentation is exerted at the level of transcription of KlPDC1. We have isolated and identified a mutation of the transcription factor KlGCR1, Klgcr1-1, which suppressed the fermentative-deficient phenotype associated with the RAG3 deletion. In the mutant, the transcription of KlPDC1 was restored. However, we found that the suppression was not specific to the RAG3 mutation, as the Klgcr1-1 mutation could also suppress the fermentative defect associated with mutation of Sck1, another regulator of glycolysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Fermentation
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal*
  • Kluyveromyces / genetics*
  • Kluyveromyces / growth & development
  • Kluyveromyces / metabolism
  • Mutation*
  • Pyruvate Decarboxylase / genetics
  • Pyruvate Decarboxylase / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription, Genetic

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • RAG3 protein, Kluyveromyces lactis
  • Transcription Factors
  • Pyruvate Decarboxylase