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Curr Biol. 2007 Jun 19;17(12):1082-9. Epub 2007 Jun 7.

Clockwork orange encodes a transcriptional repressor important for circadian-clock amplitude in Drosophila.

Author information

1
Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208, USA.

Abstract

Gene transcription is a central timekeeping process in animal clocks. In Drosophila, the basic helix-loop helix (bHLH)-PAS transcription-factor heterodimer, CLOCK/CYCLE (CLK/CYC), transcriptionally activates the clock components period (per), timeless (tim), Par domain protein 1 (Pdp1), and vrille (vri), which feed back and regulate distinct features of CLK/CYC function. Microarray studies have identified numerous rhythmically expressed transcripts, some of which are potential direct CLK targets. Here we demonstrate a circadian function for one such target, a bHLH-Orange repressor, CG17100/CLOCKWORK ORANGE (CWO). cwo is rhythmically expressed, and levels are reduced in Clk mutants, suggesting that cwo is CLK activated in vivo. cwo mutants display reduced-amplitude molecular and behavioral rhythms with lengthened periods. Molecular analysis suggests that CWO acts, in part, by repressing CLK target genes. We propose that CWO acts as a transcriptional and behavioral rhythm amplifier.

PMID:
17555964
PMCID:
PMC1963421
DOI:
10.1016/j.cub.2007.05.039
[Indexed for MEDLINE]
Free PMC Article

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