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Emerg Infect Dis. 2007 Apr;13(4):553-8.

Diagnosis of tuberculosis by an enzyme-linked immunospot assay for interferon-gamma.

Author information

1
National Taiwan University Hospital, Taipei, Taiwan, Republic of China.

Abstract

We evaluated an enzyme-linked immunospot assay for interferon-gamma (T SPOT-TB) for rapid diagnosis of active tuberculosis (TB) in a disease-endemic area. From January to June 2005, patients whose clinical symptoms and radiographic findings were compatible with TB were recruited, and a blood sample was obtained for T SPOT-TB assay within 7 days of microbiologic studies. Sixty-five patients were studied, including 39 (60%) with active TB. Thirty-five (53.8%) patients had underlying medical conditions. Thirty-seven patients had positive cultures for Mycobacterium tuberculosis, and 11 patients had positive cultures for nontuberculous mycobacteria. The sensitivity, specificity, positive predictive value, and negative predictive value of the T SPOT-TB assay were 87.2%, 88.5%, 91.9%, and 82.1%, respectively. The accuracy of this test in diagnosing active TB is >80%, even in an area with a high incidence of nontuberculous mycobacterial disease.

PMID:
17553269
PMCID:
PMC2725949
DOI:
10.3201/eid1304.051195
[Indexed for MEDLINE]
Free PMC Article

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