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Nat Methods. 2007 Jul;4(7):567-9. Epub 2007 Jun 3.

Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopy.

Author information

1
WI-MIT BioImaging Center, 500 Technology Square NE47-287, Cambridge, Massachusetts 02142, USA. bzeskind@alum.mit.edu

Abstract

We developed a deep-ultraviolet (UV) microscope capable of imaging cell mitosis and motility at 280 nm for 45 min with minimal UV-induced toxicity, and for 6 h before the onset of visible cell death in cultured human and mouse cells. Combined with computational methods that convert the intensity of each pixel into an estimate of mass, deep-UV microscopy images generate maps of nucleic acid mass, protein mass and fluorescence yield in unlabeled cells.

PMID:
17546037
DOI:
10.1038/nmeth1053
[Indexed for MEDLINE]

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