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Nat Protoc. 2007;2(6):1356-9.

Isolation of renal proximal tubular brush-border membranes.

Author information

1
Institute of Physiology, University Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland. juergbiber@access.uzh.ch

Abstract

This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.

PMID:
17545973
DOI:
10.1038/nprot.2007.156
[Indexed for MEDLINE]

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