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J Clin Microbiol. 2007 Aug;45(8):2726-30. Epub 2007 May 30.

Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach.

Author information

1
Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany. roger.dumke@mailbox.tu-dresden.de

Abstract

To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22.

PMID:
17537933
PMCID:
PMC1951254
DOI:
10.1128/JCM.00321-07
[Indexed for MEDLINE]
Free PMC Article

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