Format

Send to

Choose Destination
Mycorrhiza. 2007 Oct;17(7):571-580. doi: 10.1007/s00572-007-0134-5. Epub 2007 May 26.

Gene expression profiling of a Zn-tolerant and a Zn-sensitive Suillus luteus isolate exposed to increased external zinc concentrations.

Author information

1
Environmental Biology Group, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590, Diepenbeek, Belgium.
2
Department of Molecular Genetics and Microbiology, Duke University Medical Center, DUMC Box 3020, Durham, NC, 27710, USA.
3
Laboratoire de Physiologie et de Génétique Moléculaire des Plantes, Université Libre de Bruxelles, 1050, Brussels, Belgium.
4
Environmental Biology Group, Centre for Environmental Sciences, Hasselt University, Agoralaan, Gebouw D, 3590, Diepenbeek, Belgium. jan.colpaert@uhasselt.be.

Abstract

Complementary DNA (cDNA)-amplified fragment-length polymorphism (AFLP) was applied to analyze transcript profiles of a Zn-tolerant and a Zn-sensitive isolate of the ectomycorrhizal basidiomycete Suillus luteus, both cultured with and without increased external zinc concentrations. From the obtained transcript profiles that covered approximately 2% of the total expected complement of genes in S. luteus, 144 nonredundant, differentially expressed transcript-derived fragments (TDFs), falling in different classes of expression pattern, were isolated and sequenced. Thirty-six of the represented genes showed homology to function-known genes, whereas 6 matched unknown protein coding sequences, and 102 were possibly novel. Although relatively few TDFs were found to be responsive to the different zinc treatments, their modulated expression levels may suggest a different transcriptional response to zinc treatments in both isolates. Among the identified genes that could be related to heavy-metal detoxification or the tolerance trait were genes encoding for homologues of a heat-shock protein, a putative metal transporter, a hydrophobin, and several proteins involved in ubiquitin-dependent proteolysis.

PMID:
17530303
DOI:
10.1007/s00572-007-0134-5
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center