Abstract
The effects of an antisense oligodeoxynucleotide to codons 2-7 of the oncogene MYCN on the human neuroblastoma cell line LAN-5 were studied. Treated cells showed a decreased MYCN protein expression and synthesis by immunoperoxidase staining and immunoprecipitation. At the same time, the replication rate was inhibited, and the phenotype was modified toward a more differentiated type. Our data suggest the involvement of oncogene MYCN in both proliferative and differentiative processes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cell Differentiation / drug effects
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Cell Differentiation / genetics
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Cell Division / drug effects*
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Cell Division / genetics
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Cell Line, Transformed
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DNA Replication / drug effects
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DNA, Antisense / pharmacology*
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Gene Expression Regulation, Neoplastic / drug effects*
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Genes, myc / physiology*
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Humans
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Molecular Sequence Data
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Neurofilament Proteins / analysis
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Vimentin / analysis
Substances
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DNA, Antisense
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Neurofilament Proteins
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Vimentin