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Biophys J. 2007 Sep 15;93(6):2199-208. Epub 2007 May 18.

Nanometer-sized diamond particle as a probe for biolabeling.

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  • 1Institute of Pharmacology and Toxicology, Tzu-Chi University, Hualien, 970 Taiwan.

Abstract

A novel method is proposed using nanometer-sized diamond particles as detection probes for biolabeling. The advantages of nanodiamond's unique properties were demonstrated in its biocompatibility, nontoxicity, easily detected Raman signal, and intrinsic fluorescence from its natural defects without complicated pretreatments. Carboxylated nanodiamond's (cND's) penetration ability, noncytotoxicity, and visualization of cND-cell interactions are demonstrated on A549 human lung epithelial cells. Protein-targeted cell interaction visualization was demonstrated with cND-lysozyme complex interaction with bacteria Escherichia coli. It is shown that the developed biomolecule-cND complex preserves the original functions of the test protein. The easily detected natural fluorescent and Raman intrinsic signals, penetration ability, and low cytotoxicity of cNDs render them promising agents in multiple medical applications.

PMID:
17513352
PMCID:
PMC1959542
DOI:
10.1529/biophysj.107.108134
[PubMed - indexed for MEDLINE]
Free PMC Article
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