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Mol Cell Endocrinol. 2007 Jun 30;272(1-2):1-13. Epub 2007 Apr 6.

Relaxin signalling in THP-1 cells uses a novel phosphotyrosine-dependent pathway.

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School of Molecular and Biomedical Sciences, University of Adelaide, SA 5005, Australia.


The heterodimeric peptide hormone relaxin acts through the novel G-protein coupled receptor LGR7 to elicit the production of cAMP in the human monocyte cell line THP-1. The very small number of receptors on the cell surface, and the lack of response in cell membranes imply the involvement of a cytoplasmic signal amplification process. Here we show that this process comprises a novel and specific tyrosine kinase activity close to the receptor, and involves neither protein kinase A, mitogen-activated protein kinase, nor phosphoinositide-3 kinase activities as major upstream components. Furthermore, this novel involvement of a tyrosine kinase activity is cell-type dependent, being largely absent from LGR7-transfected HEK293T cells, and receptor-dependent; vasoactive intestinal peptide or isoproterenol signalling in the same cells does not require this tyrosine kinase activity.

[Indexed for MEDLINE]

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