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Mol Cell Biochem. 2007 Sep;303(1-2):131-9. Epub 2007 May 17.

Isolation of a novel complex of the SWI/SNF family from Schizosaccharomyces pombe and its effects on in vitro transcription in nucleosome arrays.

Author information

1
Departamento de Ciencias Biomédicas, Facultad de Medicina, Centro de Biomedicina del Norte (CBN), Universidad Católica del Norte, Larrondo 1281, Coquimbo, Chile. gbernal@ucn.cl

Abstract

The family of ATP-dependent chromatin-remodeling factors plays a central role in eukaryotic transcriptional regulation. These complexes can alter the structure of chromatin by mechanisms that involve nucleosome sliding, dissociation, or replacement over a specific promoter. The SWI/SNF chromatin-remodeling complex is required for transcriptional activation or repression in a subset of genes. In the present study we have isolated the spSWI/SNF complex from Schizosaccharomyces pombe, which has at least seven subunits among them spSwi1-like and the catalytic subunit spBrg1. These subunits are homologues to Swi1 and Swi2/Snf2, respectively in Sacharomyces cerevisiae. Moreover, we have demonstrated that spSWI/SNF is able to promote in vitro transcription by RNA polymerase II (RNAPII) in a reconstituted system. In our transcription assays with cellular extracts of Sc. pombe we did not observe inhibition when alpha-Swi1 antibodies were utilized, indicating that other chromatin-remodeling complexes may allow transcription in Sc. pombe.

PMID:
17508131
DOI:
10.1007/s11010-007-9465-y
[Indexed for MEDLINE]

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