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Phys Rev Lett. 2007 Mar 23;98(12):128105. Epub 2007 Mar 21.

Exploring the mechanism of flexible biomolecular recognition with single molecule dynamics.

Author information

1
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People's Republic of China.

Abstract

Combining a single-molecule study of protein binding with a coarse grained molecular dynamics model including solvent (water molecules) effects, we find that biomolecular recognition is determined by flexibilities in addition to structures. Our single-molecule study shows that binding of CBD (a fragment of Wiskott-Aldrich syndrome protein) to Cdc42 involves bound and loosely bound states, which can be quantitatively explained in our model as a result of binding with large conformational changes. Our model identified certain key residues for binding consistent with mutational experiments. Our study reveals the role of flexibility and a new scenario of dimeric binding between the monomers: first bind and then fold.

PMID:
17501161
DOI:
10.1103/PhysRevLett.98.128105
[Indexed for MEDLINE]

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