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J Agric Food Chem. 2007 Jun 13;55(12):4651-7. Epub 2007 May 12.

High throughput analysis of red wine and grape phenolics-adaptation and validation of methyl cellulose precipitable tannin assay and modified Somers color assay to a rapid 96 well plate format.

Author information

1
The Australian Wine Research Institute, P.O. Box 197, Glen Osmond, SA, 5064, Australia.

Abstract

The methyl cellulose precipitable (MCP) tannin assay and a modified version of the Somers and Evans color assay were adapted to high-throughput (HTP) analysis. To improve efficiency of the MCP tannin assay, a miniaturized 1 mL format and a HTP format using 96 well plates were developed. The Somers color assay was modified to allow the standardization of pH and ethanol concentrations of wine samples in a simple one-step dilution with a buffer solution, thus removing inconsistencies between wine matrices prior to analysis and allowing for its adaptation to a HTP format. Validation studies showed that all new formats were efficient, and results were reproducible and analogous to the original formats.

PMID:
17497877
DOI:
10.1021/jf063674n
[Indexed for MEDLINE]

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