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Avian Dis. 2007 Mar;51(1 Suppl):393-5.

Development of a reverse genetics system enabling the rescue of recombinant avian influenza virus A/Turkey/England/50-92/91 (H5N1).

Author information

1
School of Biological Sciences, University of Reading, Berkshire RG6 6AJ, United Kingdom.

Abstract

We previously described the use of an established reverse genetics system for the generation of recombinant human influenza A viruses from cloned cDNAs. Here, we have assembled a set of plasmids to allow recovery of the avian H5N1 influenza virus A/Turkey/England/50-92/91 entirely from cDNA. This system enables us to introduce mutations or truncations into the cDNAs to create mutant viruses altered specifically in a chosen gene. These mutant viruses can then be used in future pathogenesis studies in chickens and in studies to understand the host range restrictions of avian influenza viruses in humans.

PMID:
17494592
DOI:
10.1637/7645-050906R1.1
[Indexed for MEDLINE]

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