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J Biomed Opt. 2007 Mar-Apr;12(2):020505.

Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells.

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1
University of California-Irvine, Beckman Laser Institute, Irvine, California 92612, USA.

Abstract

An 800-nm 200-fs laser is used to produce DNA damage in rat kangaroo (PtK1) and human cystic fibrosis pancreatic adenoma carcinoma (CFPAC-1) cells. Immunofluorescence staining for DNA repair factors in irradiated cells displays localization of gammaH2AX, Nbs1, and Rad50 to the site of irradiation 3 to 30 min following laser exposure. It is concluded that the 200-fs near-infrared laser is an excellent source for the production and study of spatially defined regions of DNA damage.

PMID:
17477704
DOI:
10.1117/1.2717684
[Indexed for MEDLINE]
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