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Proteomics. 2007 Jun;7(11):1746-52.

iTRAQ compatibility of peptide immobilized pH gradient isoelectric focusing.

Author information

1
Karolinska Biomic Center, Karolinska University Hospital, Stockholm, Sweden.

Abstract

Immobilized pH gradient isoelectric focusing (IPG-IEF) has emerged as a highly promising alternative to strong-cation exchange fractionation as the first dimension in shot-gun proteomics. Herein is shown the compatibility of this method with iTRAQ isotope labeling for relative quantitation and validation of sequence matches from database searching.

PMID:
17476709
DOI:
10.1002/pmic.200600782
[Indexed for MEDLINE]

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