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Mass Spectrom Rev. 2007 Sep-Oct;26(5):657-71.

Microwave-assisted proteomics.

Author information

1
Protein Chemistry Department, Genentech Inc, South San Francisco, CA 94080, USA. JLill@gene.com

Abstract

State-of-the-art proteomic analysis has recently undergone a rapid evolution; with more high-throughput analytical instrumentation and informatic tools available, sample preparation is becoming one of the rate-limiting steps in protein characterization workflows. Recently several protocols have appeared in the literature that employ microwave irradiation as a tool for the preparation of biological samples for subsequent mass spectrometric characterization. Techniques for microwave-assisted bio-catalyzed reactions (including sample reduction and alkylation, enzymatic and chemical digestion, removal and analysis of post-translational modifications and characterization of enzymes and protein-interaction sites) are described. This review summarizes the various approaches undertaken, instrumentation employed, and reduction in overall experimental time observed when microwave assistance is applied.

PMID:
17474122
DOI:
10.1002/mas.20140
[Indexed for MEDLINE]

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