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J Magn Reson. 2007 Jul;187(1):163-9. Epub 2007 Apr 13.

A set of BEST triple-resonance experiments for time-optimized protein resonance assignment.

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Institut de Biologie Structurale, Jean-Pierre Ebel Centre National de la Recherche Scientifique-Commissariat à l'Energie Atomique-UJF, 41 rue Jules Horowitz, 38027 Grenoble Cedex, France.


A series of sequential, intra-residue, and bi-directional BEST H-N-CA, H-N-CO, and H-N-CB pulse sequences is presented that extends the BEST concept introduced recently for fast multidimensional protein NMR [Schanda et al., J. Am. Chem. Soc. 128 (2006) 9042] to the complete set of experiments required for sequential resonance assignment. We demonstrate for the protein ubiquitin that 3D BEST H-N-C correlation spectra can be recorded on a 600MHz NMR spectrometer equipped with a cryogenic probe in only a few minutes of acquisition time with sufficient sensitivity to detect all expected cross peaks.

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