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Fertil Steril. 2007 Oct;88(4 Suppl):1143-9. Epub 2007 Apr 30.

In vitro evidence of glucose-induced toxicity in GnRH secreting neurons: high glucose concentrations influence GnRH secretion, impair cell viability, and induce apoptosis in the GT1-1 neuronal cell line.

Author information

1
Department of Obstetrics, Gynecology & Women's Health, Albert Einstein College of Medicine, Bronx, New York, USA. lubnapal@aol.com

Abstract

OBJECTIVE:

To evaluate for direct toxic effects of high glucose concentrations on cellular physiology in GnRH secreting immortalized GT1-1 neurons.

DESIGN:

Prospective experimental design.

SETTING:

In vitro experimental model using a cell culture system.

INTERVENTION(S):

GT1-1 cells were cultured in replicates in media with two different glucose concentrations (450 mg/dL and 100 mg/dL, respectively) for varying time intervals (24, 48, and 72 hours).

MAIN OUTCOME MEASURE(S):

Effects of glucose concentrations on GnRH secretion by the GT1-1 neurons were evaluated using a static culture model. Cell viability, cellular apoptosis, and cell cycle events in GT1-1 neurons maintained in two different glucose concentrations were assessed by flow cytometry (fluorescence-activated cell sorter) using Annexin V-PI staining.

RESULT(S):

Adverse influences of high glucose concentrations on GnRH secretion and cell viability were noted in cultures maintained in high glucose concentration (450 mg/dL) culture medium for varying time intervals. A significantly higher percentage of cells maintained in high glucose concentration medium demonstrated evidence of apoptosis by a fluorescence-activated cell sorter.

CONCLUSION(S):

We provide in vitro evidence of glucose-induced cellular toxicity in GnRH secreting GT1-1 neurons. Significant alterations in GnRH secretion, reduced cell viability, and a higher percentage of apoptotic cells were observed in GT1-1 cells maintained in high (450 mg/dL) compared with low (100 mg/dL) glucose concentration culture medium.

PMID:
17466987
PMCID:
PMC2211508
DOI:
10.1016/j.fertnstert.2007.01.007
[Indexed for MEDLINE]
Free PMC Article

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