The effects of tripeptide corticoliberin fragment CRF4-6 (Pro-Pro-Ile) on blood glucose level and the rat body temperatire were investigated. Intracerebroventricularly injected CRF4-6 (6, 30, 150 nmol/head) causes a dosedependent hyperglycemia and hyperthermia in anaesthetized animals. Corticotropin releasing factor antagonist alpha-helical CRF4-6 (6.5 nmol/head) abolishes the influence of tripeptide CRF4-6 (6 nmol/head) on blood glucose level and body temperature of rats. Bilateral adrenalectomy has no effect on tripeptide-induced hyperglycemia and hyperthemia. This result indicates that hyperglycemic and hyperthermal effects of tripeptide occur independently of adrenal gland catecholamines. In addition, non-pituitary corticoliberin receptors are involved in CRF4-6 influences on blood glucose level and body temperature.