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Nat Biotechnol. 2007 May;25(5):566-8. Epub 2007 Apr 22.

Quantitative proteomic assessment of very early cellular signaling events.

Author information

1
Center of Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Campusvej 55, DK-5230, Odense, Denmark.

Abstract

Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.

PMID:
17450129
DOI:
10.1038/nbt1301
[Indexed for MEDLINE]

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