Format

Send to

Choose Destination
Biochemistry (Mosc). 2007 Mar;72(3):328-31.

"False" thymine-1H-Enol guanine base pair. low misinsertion rate by DNA polymerase explained by computational chemistry consideration.

Author information

1
University of Medicine and Pharmacy, Victor Babes Timisoara, Timisoara, Romania. eddie@acad-icht.tm.edu.ro

Abstract

Formation of correct TA and GC and "false" thymine-1H-enol guanine (TGenol) base pairs is here considered to control nucleotide insertion into DNA via low substrate concentration Michaelis-Menten controlled kinetics. Contributions of base pairing to formation of Gibbs free energies in water solution, DeltaDeltaG, are calculated for the correct and false base pairs with the semi-empiric MNDO/PM3 method for base pairing energies in vacuum and the BEM method for hydration effects. The results for DeltaDeltaG indicate equal insertion rates for correct base pairing and a 10(-3)-10(-4) error probability for false insertion controlled by the TGenol false pair.

PMID:
17447887
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center