Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Protoc. 2007;2(4):875-7.

Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent.

Author information

1
USDA ARS Photosynthesis Research Unit, Urbana, Illinois 61801, USA. ainswort@uiuc.edu

Abstract

Non-structural phenolic compounds perform a variety of functions in plants, including acting as antioxidants. We describe a microplate-adapted colorimetric total phenolics assay that utilizes Folin-Ciocalteu (F-C) reagent. The F-C assay relies on the transfer of electrons in alkaline medium from phenolic compounds to phosphomolybdic/phosphotungstic acid complexes, which are determined spectroscopically at 765 nm. Although the electron transfer reaction is not specific for phenolic compounds, the extraction procedure eliminates approximately 85% of ascorbic acid and other potentially interfering compounds. This assay is performed in microcentrifuge tubes and assessed in a 96-well plate reader. At least 64 samples can be processed in 1 d.

PMID:
17446889
DOI:
10.1038/nprot.2007.102
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Support Center