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Int Immunol. 2007 May;19(5):675-84. Epub 2007 Apr 19.

Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement.

Author information

1
Competence Centre for Biomolecular Therapeutics Research Vienna, A-1090, Vienna.

Abstract

The existence of lipid rafts and their importance for immunoreceptor signaling is highly debated. By non-invasive single molecule imaging, we analyzed the dynamics of the T-cell antigen receptor (TCR), the lipid raft-associated glycosylphosphatidylinositol (GPI) proteins CD48 and CD59 and the major leukocyte phosphatase CD45 in living naive T lymphocytes. TCR triggering induced the immobilization of CD45 and CD48 at different positions within the T-cell interface. The second GPI protein, CD59, did not co-immobilize indicating lipid raft heterogeneity in living T lymphocytes. A novel biochemical approach confirmed that lipid raft components are not associated in the plasma membrane of resting cells, and variably associate with specific receptors to distinct lipid rafts upon activation.

PMID:
17446208
DOI:
10.1093/intimm/dxm032
[Indexed for MEDLINE]

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