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Res Vet Sci. 2007 Dec;83(3):340-6. Epub 2007 Apr 17.

Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis.

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Departamento de Patologia e Clínicas, Escola de Medicina Veterinária, Universidade Federal da Bahia, Av. Adhemar de Barros, 500 Salvador, BA, CEP 40170-110, Brazil.


An indirect ELISA test was developed for the diagnosis of Brucella canis infection in dogs. A bacterial whole cell extract was used as a solid phase antigen, using B. canis isolated from an infected animal. Sera from culture-positive and healthy negative animals were used as internal reference controls. The cut-off point was determined by a mathematical formula for a statistically valid value, which defined the upper prediction limit, based on the upper tail of the t-distribution of 21 negative control sera readings, for the confidence level of 99.5%. The sensitivity and specificity of the ELISA test were 95% and 91%, respectively. The ELISA test showed a significant concordance index (K=0.84) with the agar gel immunodiffusion test. The reliability of the ELISA for the detection of infected animals was established by a double blind study testing 280 sera provided by serum banks from different diagnostic and research institutions and analyzed by ROC Curve.

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