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Biochem Biophys Res Commun. 2007 Jun 8;357(3):668-71. Epub 2007 Apr 10.

The R1441C mutation of LRRK2 disrupts GTP hydrolysis.

Author information

1
Laboratory of Neurogenetics, National Institute on Aging, 35 Convent Drive, Bethesda, MD 20892-3707, USA.

Abstract

Mutations in Leucine Rich Repeat Kinase 2 (LRRK2) are the leading genetic cause of Parkinson's disease (PD). LRRK2 is predicted to contain kinase and GTPase enzymatic domains, with recent evidence suggesting that the kinase activity of LRRK2 is central to the pathogenic process associated with this protein. The GTPase domain of LRRK2 plays an important role in the regulation of kinase activity. To investigate how the GTPase domain might be related to disease, we examined the GTP binding and hydrolysis properties of wild type and a mutant form of LRRK2. We show that LRRK2 immunoprecipitated from cells has a detectable GTPase activity that is disrupted by a familial mutation associated with PD located within the GTPase domain, R1441C.

PMID:
17442267
PMCID:
PMC1939973
DOI:
10.1016/j.bbrc.2007.04.006
[Indexed for MEDLINE]
Free PMC Article

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