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J Proteome Res. 2007 Jun;6(6):2205-10. Epub 2007 Apr 19.

Increased coverage in the transmembrane domain with activated-ion electron capture dissociation for top-down Fourier-transform mass spectrometry of integral membrane proteins.

Author information

1
Thermo Fisher, San Jose, California, California 95134, USA.

Abstract

The c-subunit of ATP synthase (AtpH) is an 8 kD integral membrane protein with two transmembrane domains; we set out to demonstrate it amenable to top-down electrospray-ionization Fourier-transform mass spectrometry (FT-MS) using both collision activated and electron capture dissociation (CAD/ECD). Thermal activation concomitant with electron delivery was necessary for efficient ECD (activated-ion ECD; aiECD), yielding complementary information and greater sequence coverage in the transmembrane domains in comparison with CAD.

PMID:
17441748
DOI:
10.1021/pr0607031
[Indexed for MEDLINE]

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