The effect of basketball training on the players' erythrocyte membrane acetylcholinesterase, (Na+,K+)-ATPase and Mg2+-ATPase activities

Int J Sports Med. 2007 Aug;28(8):650-4. doi: 10.1055/s-2007-964855. Epub 2007 Apr 13.

Abstract

The aim of this study was to investigate whether the activities of erythrocyte membrane acetylcholinesterase (AChE), (Na (+),K (+))-ATPase and Mg (2+)-ATPase are modulated by a basketball training. Blood was obtained from 10 basketball players pre- and postexercise. Total antioxidant status (TAS), lactate and pyruvate concentrations were determined with kits, while the enzyme activities were determined spectrophotometrically. Post-training blood lactate and pyruvate concentrations as well as AChE (2.90 +/- 0.05 vs. 3.98 +/- 0.09 Delta OD/min . mg protein, p < 0.01) and Na (+),K (+)-ATPase (0.58 +/- 0.04 vs. 1.27 +/- 0.12 micromol Pi/h . mg protein, p < 0.001) activities were remarkably increased, whereas TAS was significantly decreased. Mg (2+)-ATPase activity remained unaltered at the end of the training. In conclusion, the stimulation of AChE and Na (+),K (+)-ATPase by the training may be due to the rise of blood catecholamine oxidation contributing to TAS decrease and/or the increase of serotonin levels. This stress condition may modulate cholinergic and catecholaminergic/serotoninergic functions in players.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholinesterase / metabolism*
  • Adolescent
  • Antioxidants / analysis
  • Antioxidants / metabolism
  • Basketball*
  • Ca(2+) Mg(2+)-ATPase / metabolism*
  • Erythrocyte Membrane / enzymology*
  • Exercise / physiology
  • Greece
  • Humans
  • Male
  • Oxidative Stress
  • Sodium-Potassium-Exchanging ATPase / metabolism*

Substances

  • Antioxidants
  • Acetylcholinesterase
  • Ca(2+) Mg(2+)-ATPase
  • Sodium-Potassium-Exchanging ATPase