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Trends Biotechnol. 2007 Jun;25(6):247-9. Epub 2007 Apr 12.

Catalytic efficiency and kcat/KM: a useful comparator?

Author information

1
Department of Biology & Biochemistry, University of Bath, Bath, UK.

Abstract

The ratio k(cat)/K(M)--often referred to as the "specificity constant"--is a useful index for comparing the relative rates of an enzyme acting on alternative, competing substrates. However, an alternative description, "catalytic efficiency", is frequently used, and on occasions misused, to compare the reactivity of two enzymes acting on the same substrate. Here, we highlight the pitfalls in using k(cat)/K(M) to compare the catalytic effectiveness of enzymes.

PMID:
17433847
DOI:
10.1016/j.tibtech.2007.03.010
[Indexed for MEDLINE]

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