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BMC Dev Biol. 2007 Apr 10;7:31.

Stromal derived factor-1 exerts differential regulation on distinct cortical cell populations in vitro.

Author information

1
1,124 Stopford Building, Faculty of Life Sciences, The University of Manchester, Manchester, UK. J.Pritchett@postgrad.manchester.ac.uk <J.Pritchett@postgrad.manchester.ac.uk>

Abstract

BACKGROUND:

Stromal derived factor (SDF-1), an alpha chemokine, is a widely known chemoattractant in the immune system. A growing body of evidence now suggests multiple regulatory roles for SDF-1 in the developing nervous system.

RESULTS:

To investigate the role of SDF-1 signaling in the growth and differentiation of cortical cells, we performed numerous in vitro experiments, including gene chip and quantitative RT-PCR analysis. Using SDF-1 medium and AMD3100, a receptor antagonist, we demonstrate that the chemokine signaling regulates key events during early cortical development. First, SDF-1 signaling maintains cortical progenitors in proliferation, possibly through a mechanism involving connexin 43 mediated intercellular coupling. Second, SDF-1 signaling upregulates the differentiation of cortical GABAergic neurons, independent of sonic signaling pathway. Third, SDF-1 enables the elongation and branching of axons of cortical glutamatergic neurons. Finally, cortical cultures derived from CXCR4-/- mutants show a close parallel to AMD3100 treatment with reduced cell proliferation and differentiation of GABAergic neurons.

CONCLUSION:

Results from this study show that SDF-1 regulates distinct cortical cell populations in vitro.

PMID:
17425785
PMCID:
PMC1854892
DOI:
10.1186/1471-213X-7-31
[Indexed for MEDLINE]
Free PMC Article

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