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Methods Mol Biol. 2007;362:309-14.

Extraction of plant RNA.

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1
Department of Biology, University of Leicester, UK.

Abstract

This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.

PMID:
17417018
DOI:
10.1007/978-1-59745-257-1_21
[Indexed for MEDLINE]
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