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Pancreas. 2007 Apr;34(3):310-7.

Plasma proteomic analysis of pancreatic cancer by 2-dimensional gel electrophoresis.

Author information

1
Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, PR China.

Abstract

OBJECTIVE:

Late diagnosis is the major reason for poor prognosis of pancreatic cancer (PC). Developing new biomarkers of early stage detection is critical.

METHODS:

Proteomic analysis with 2-dimensional gel electrophoresis was used to identify differentially expressed proteins in plasma of PC patients. The 2-dimensional gel electrophoresis plasma protein profiles of 11 PC patients (preoperative and postoperative) were compared with those of 10 patients with chronic pancreatitis (CP) and 11 healthy controls.

RESULTS:

Five proteins were found to be constantly changed. Haptoglobin (Hp) beta chain and leucine-rich alpha2 glycoprotein up-regulated slightly in PC plasma. Pancreatic cancer had a higher frequency of Hp2-2 phenotype but lacked Hp1-1 phenotype. Hemoglobin was increased significantly in plasma samples of PC and CP. Alpha1 antitrypsin gradually increased its expression level in healthy control, PC, and CP. Immunoglobin J chain was elevated in CP plasma samples. Haptoglobin, alpha1 antitrypsin, and leucine-rich alpha2-glycoprotein were all greatly elevated after tumor resection in PC patients.

CONCLUSIONS:

Proteomic analysis can simultaneously detect changes of multiproteins in plasma of PC, but detected proteins are abundant and common plasma proteins and their diagnostic value may be limited.

PMID:
17414053
DOI:
10.1097/MPA.0b013e31802f2483
[Indexed for MEDLINE]

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