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N Engl J Med. 2007 Apr 5;356(14):1410-22.

Invariant natural killer T cells in asthma and chronic obstructive pulmonary disease.

Author information

1
Division of Infection, Inflammation, and Repair, University of Southampton, Southampton General Hospital, Southampton, United Kingdom. vijay@soton.ac.uk

Abstract

BACKGROUND:

The number of type 2 helper CD4+ T cells is increased in the airways of persons with asthma. Whether the majority of these cells are class II major-histocompatibility-complex-restricted cells or are among the recently identified CD1d-restricted invariant natural killer T cells is a matter of controversy. We studied the frequency of invariant natural killer T cells in the airways of subjects with mild or moderately severe asthma to investigate the possibility of an association between the number of invariant natural killer T cells in the airway and disease severity. We also studied whether an increased number of these cells is a feature of chronic obstructive pulmonary disease (COPD).

METHODS:

We enumerated invariant natural killer T cells by flow cytometry with the use of CD1d tetramers loaded with alpha-galactosylceramide and antibodies specific to the invariant natural killer T-cell receptor in samples of bronchoalveolar-lavage fluid, induced sputum, and bronchial-biopsy specimens obtained from subjects with mild or moderately severe asthma, subjects with COPD, and healthy control subjects. Real-time polymerase-chain-reaction analysis was performed on bronchoalveolar-lavage cells for evidence of gene expression of the invariant natural killer T-cell receptor.

RESULTS:

Fewer than 2% of the T cells obtained from all subjects on airway biopsy, bronchoalveolar lavage, and sputum induction were invariant natural killer T cells, with no significant differences among the three groups of subjects. No expression of messenger RNA for the invariant natural killer T-cell-receptor domains Valpha24 and Vbeta11 was detected in bronchoalveolar-lavage cells from subjects with asthma.

CONCLUSIONS:

Invariant natural killer T cells are found in low numbers in the airways of subjects with asthma, subjects with COPD, and controls.

PMID:
17409322
DOI:
10.1056/NEJMoa064691
[Indexed for MEDLINE]

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