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Chromosome Res. 2007;15(3):361-70. Epub 2007 May 10.

Integrated cytogenetic BAC map of the genome of the gray, short-tailed opossum, Monodelphis domestica.

Author information

1
Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 4700 Hillsborough Street, North Carolina State University, Raleigh, NC 27606, USA.

Abstract

The generation of high-quality genome assemblies for numerous species is advancing at a rapid pace. As the number of genome assemblies increases, so does our ability to investigate genome relationships and their contributions to unraveling complex biological, evolutionary, and biomedical processes. A key process in the generation of a genome assembly is to determine and verify the precise physical location and order of the large sequence blocks (scaffolds) that result from the assembly. For organisms of relatively recent common ancestry this process may be achieved largely through comparative sequence alignment. However, as the evolutionary distance between species lengthens, the use of comparative sequence alignment becomes increasingly less reliable. Simultaneous cytogenetic mapping, using multicolor fluorescence in-situ hybridization (FISH) analysis, offers an alternative means to define the cytogenetic location and relative order of DNA sequences, thereby anchoring the genome sequence to the karyotype. In this article we report the molecular cytogenetic locations of 415 bacterial artificial chromosome (BAC) clones that served to anchor sequence scaffolds of the gray, short-tailed opossum (Monodelphis domestica) to its karyotype, which enabled accurate integration of these regions into the genome assembly.

PMID:
17406991
DOI:
10.1007/s10577-007-1131-4
[Indexed for MEDLINE]

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