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PLoS One. 2007 Apr 4;2(4):e358.

Modular mass spectrometric tool for analysis of composition and phosphorylation of protein complexes.

Author information

1
Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, United States of America.

Abstract

The combination of high accuracy, sensitivity and speed of single and multiple-stage mass spectrometric analyses enables the collection of comprehensive sets of data containing detailed information about complex biological samples. To achieve these properties, we combined two high-performance matrix-assisted laser desorption ionization mass analyzers in one modular mass spectrometric tool, and applied this tool for dissecting the composition and post-translational modifications of protein complexes. As an example of this approach, we here present studies of the Saccharomyces cerevisiae anaphase-promoting complexes (APC) and elucidation of phosphorylation sites on its components. In general, the modular concept we describe could be useful for assembling mass spectrometers operating with both matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) ion sources into powerful mass spectrometric tools for the comprehensive analysis of complex biological samples.

PMID:
17406682
PMCID:
PMC1832223
DOI:
10.1371/journal.pone.0000358
[Indexed for MEDLINE]
Free PMC Article

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