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Nat Protoc. 2006;1(6):2856-60.

In-gel digestion for mass spectrometric characterization of proteins and proteomes.

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1
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany. shevchenko@mpi-cbg.de

Abstract

In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.

PMID:
17406544
DOI:
10.1038/nprot.2006.468
[Indexed for MEDLINE]
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