Format

Send to

Choose Destination
Nat Protoc. 2006;1(5):2227-30.

A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii.

Author information

1
Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA.

Abstract

This method has been developed to yield highly purified intact chloroplasts from Chlamydomonas reinhardtii. This procedure involves breaking cell-wall-deficient cells by passage through a narrow-bore syringe needle and purifying the intact chloroplasts by differential centrifugation and Percoll gradient centrifugation. This procedure can be completed in less than 3 h and is capable of generating relatively high yields of chloroplasts that should be useful for researchers studying the biochemistry and cell biology of C. reinhardtii chloroplasts.

PMID:
17406461
DOI:
10.1038/nprot.2006.348
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center