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Clin Microbiol Infect. 2007 Jul;13(7):725-7. Epub 2007 Apr 2.

A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I-V.

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1
Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark. Kit.Boye@hvh.regionh.dk

Abstract

A multiplex PCR with four primer-pairs was designed to identify the five main known SCCmec types. A clear and easily discriminated band pattern was obtained for all five types. The SCCmec type was identified for 98% of 312 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). SCCmec type IV was by far the most common SCCmec type among both hospital- and community-acquired MRSA isolates in Denmark.

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