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Nat Protoc. 2007;2(1):38-41.

Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.

Author information

1
Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.

Abstract

Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.

PMID:
17401336
DOI:
10.1038/nprot.2007.15
[Indexed for MEDLINE]

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