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Nat Protoc. 2007;2(1):31-4.

High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.

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1
Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.

Abstract

Here we describe a high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae. This method currently gives the highest efficiency and yield of transformants, although a faster protocol is available for small number of transformations. The procedure takes up to 1.5 h, depending on the length of heat shock, once the yeast culture has been grown. This method is useful for most transformation requirements.

PMID:
17401334
DOI:
10.1038/nprot.2007.13
[Indexed for MEDLINE]
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