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J Virol Methods. 2007 Jul;143(1):81-5. Epub 2007 Mar 29.

Implementation of a one-step real-time RT-PCR protocol for diagnosis of foot-and-mouth disease.

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Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, United Kingdom.


An automated one-step real-time reverse transcription polymerase chain reaction (rRT-PCR) protocol was optimised and evaluated for the routine diagnosis of foot-and-mouth disease (FMD). Parallel testing of RNA samples (n=257) indicated that this assay has a diagnostic sensitivity at least equivalent to the automated two-step rRT-PCR protocol previously used for the laboratory detection of FMD virus (FMDV). This more rapid and economical one-step protocol will play a key role in contingency planning for any future outbreaks of FMD in the United Kingdom (UK).

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