Parts of leaves, stems and roots of Ruta graveolens ssp. divaricata (Tenore) Gams were explanted on Linsmaier and Skoog (16) medium. All explants showed good callus growth under various culture conditions. With stem derived tissue cultures, the capability for differentiation and regeneration was examined in response to variations of media and phytohormonal composition. In darkness, cultures produced roots of various length depending on composition of the media. Under continuous illumination (1200 lux) cultures produced fully green shoot regenerates on various media though complete plants could not be regenerated.