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Nano Lett. 2007 Apr;7(4):970-7. Epub 2007 Mar 28.

Ligand-conjugated quantum dots monitor antigen uptake and processing by dendritic cells.

Author information

1
Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany. a.cambi@ncmls.ru.nl

Abstract

The dendritic cell (DC) specific pathogen-uptake receptor (DC-SIGN) internalizes antigens for degradation and presentation onto MHC molecules. At the cell membrane, DC-SIGN forms nanoclusters that facilitate virus capture. However, internalized viruses, such as HIV-1, escape degradation. Here, we exploit ligand-conjugated, virus-sized, highly photostable quantum dots (QDs) to monitor in living cells antigen binding, entry, and trafficking. The antigen-coated QDs specific uptake and persistence in live DCs open the possibility for tracking antigen-presenting cells in vivo.

PMID:
17388641
DOI:
10.1021/nl0700503
[Indexed for MEDLINE]

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