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FEBS Lett. 2007 Apr 17;581(8):1549-54. Epub 2007 Mar 15.

Different obscurin isoforms localize to distinct sites at sarcomeres.

Author information

1
Department of Physiology, University of Maryland School of Medicine, 655 W. Baltimore St, MD 21201, USA.

Abstract

We used four antibodies to regions of obscurin isoforms A and B, encoded by the obscurin gene, to investigate the location of these proteins in skeletal myofibers at resting and stretched lengths. Obscurin A ( approximately 800 kDa) which was recognized by antibodies generated to the N-terminal, Rho-GEF, and the non-modular C-terminal domain that lacks the kinase-like domains, localizes at the level of the M-band. Obscurin B ( approximately 900 kDa) which has the N-terminal, Rho-GEF, and the C-terminal kinase-like domains, localizes at the level of the A/I junction. Additional isoforms, which lack one or more of these epitopes, are present at the Z-disk and Z/I junction.

PMID:
17382936
PMCID:
PMC1899168
DOI:
10.1016/j.febslet.2007.03.011
[Indexed for MEDLINE]
Free PMC Article

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