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J Antimicrob Chemother. 1992 Jan;29(1):19-25.

Uptake of minocycline by Escherichia coli.

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Department of Microbial Biochemistry and Genetics, Lederle Laboratories, Pearl River, New York 10965.


Uptake of tetracyclines into Escherichia coli was assessed with a strain carrying a tetA-lacZ translational fusion, in which expression of the enzyme is controlled by the pSC101 tetR repressor gene, by examining beta-galactosidase induction. The ability of tetracycline analogues to induce beta-galactosidase synthesis was correlated with their hydrophobicity, such that hydrophobic analogues were poor enzyme inducers. Treatment of E. coli with polymyxin B nonapeptide (PMBN) rendered cells more permeable to minocycline, but not to tetracycline.

[Indexed for MEDLINE]

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