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J Proteome Res. 2007 May;6(5):2005-10. Epub 2007 Mar 22.

15N metabolic labeling of mammalian tissue with slow protein turnover.

Author information

1
Department of Cell Biology, 10550 North Torrey Pines Road, SR11, The Scripps Research Institute, LaJolla, California 92037, USA.

Abstract

We previously reported the metabolic 15N labeling of a rat where enrichment ranged from 94% to 74%. We report here an improved labeling strategy which generates 94% 15N enrichment throughout all tissues of the rat. A high 15N enrichment of the internal standard is necessary for accurate quantitation, and thus, this approach will allow quantitative mass spectrometry analysis of animal models of disease targeting any tissue.

PMID:
17375949
PMCID:
PMC2527585
DOI:
10.1021/pr060599n
[Indexed for MEDLINE]
Free PMC Article

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