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Biotechniques. 2007 Feb;42(2):137, 139, 141 passim.

Application of protein-fragment complementation assays in cell biology.

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1
Département de Biochimie, Université de Montréal, Succursale Center-Ville, Montréal, QC, Canada.

Abstract

We have developed a general experimental strategy that enables the quantitative detection of dynamic protein-protein interactions in intact living cells, based on protein-fragment complementation assays (PCAs). In this method, protein-protein interactions are coupled to refolding of enzymes from cognate fragments where reconstitution of enzyme activity acts as the detector of a protein interaction. Here we discuss the application of PCA to different aspects of cell biology.

PMID:
17373475
[Indexed for MEDLINE]
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