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Emerg Infect Dis. 2007 Jan;13(1):73-81.

Panmicrobial oligonucleotide array for diagnosis of infectious diseases.

Author information

1
Columbia University, New York, New York 10032, USA.

Abstract

To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005.

PMID:
17370518
PMCID:
PMC2725825
DOI:
10.3201/eid1301.060837
[Indexed for MEDLINE]
Free PMC Article

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